D7777

Sigma

 

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate., powder, cell culture tested

Synonym:DME, DMEM

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Description

ApplicationDulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
QuantityFormulated to contain 13.5 grams of powder per liter of medium.
ReconstitutionSupplement with 3.7 g/L sodium bicarbonate

Properties

formpowder
suitabilitycell culture tested
storage temp.2-8°C

Safety

Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36
WGK Germany1